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Long Adapter Single Stranded Oligonucleotide (LASSO) Probes for Massively Multiplexed Cloning of Kilobase-Sized Genome Regions
Manage episode 340229621 series 3391756
Вміст надано Bitesize Bio. Весь вміст подкастів, включаючи епізоди, графіку та описи подкастів, завантажується та надається безпосередньо компанією Bitesize Bio або його партнером по платформі подкастів. Якщо ви вважаєте, що хтось використовує ваш захищений авторським правом твір без вашого дозволу, ви можете виконати процедуру, описану тут https://uk.player.fm/legal.
In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn:
The difference and benefits of LASSO cloning over NGS
How LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomes
How this technology can impact our understanding of disease, drug discovery, precision medicine, and so much more
Join Dr. Lorenzo Tosi as he describes LASSO cloning, a technique that will allow for the expression of an entire set of proteins from any organism in more rapid and cost-effective manner.
The development of tools and techniques designed to sequence and understand genomes has advanced our understanding of the world at large. However, our ability to analyze the function of expressed molecules from these genomes is lagging. Even with the advent of next generation sequencing (NGS), which has changed the landscape of genomic sequencing through increased accuracy and speed, our experiments are still limited by the length of DNA targets being read.
For more information visit: https://bitesizebio.com/webinar/long-adapter-single-stranded-oligonucleotide-lasso-probes-for-massively-multiplexed-cloning-of-kilobase-sized-genome-regions/
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