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Designing Flanking Homology DNA Assembly Experiments
Manage episode 340229623 series 3391756
Вміст надано Bitesize Bio. Весь вміст подкастів, включаючи епізоди, графіку та описи подкастів, завантажується та надається безпосередньо компанією Bitesize Bio або його партнером по платформі подкастів. Якщо ви вважаєте, що хтось використовує ваш захищений авторським правом твір без вашого дозволу, ви можете виконати процедуру, описану тут https://uk.player.fm/legal.
Join us in this webinar on demystifying DNA assembly. In this webinar, you will learn:
– How flanking homology DNA assembly methods work
– How to use web-based software to design experimental methods for flanking homology DNA assembly methods
– How synthetic DNA fragments fit in to the DNA assembly process
Dr. Nathan Hillson will discuss methods in flanking homology DNA assembly, including Gibson, In-Fusion, and yeast TAR assembly—amongst many other related methods. Current DNA assembly methods offer many advantages over traditional (multiple cloning site, digestion/ligation) approaches, including the ability to assemble multiple fragments at once, the lack of a necessary specific restriction enzyme, and time commitment.
One part of DNA assembly is designing these experimental methods. To that end, Dr. Hillson will provide a demonstration of how to use web-based software to automate and optimize the design of protocols for these methods.
Finally, Dr. Hillson will explain how synthetic DNA fragments fit in to the DNA assembly process and how this relates to your work.
For more information visit: https://bitesizebio.com/webinar/flanking-homology-dna-assembly-protocol-design-software-synthetic-dna/
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