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The Listen In podcast from Bitesize Bio is a compilation of our best webinars to enjoy at your leisure, wherever and whenever. Each episode is an opportunity to gain the valuable insights you need to advance your research. From a crash course in developing fool-proof ELISAs to the latest applications and innovations in CRISPR/Cas9 and microscopy techniques, and much more—you'll hear about challenges encountered and discover practical solutions to achieve the best possible results. Tap into t ...
 
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Although macrophages were first described by Elie Metchnikoff in 1882, plenty of mysteries are still associated with the cell type. Indeed, while macrophages were once considered simply “garbage trucks” of the immune system due to their phagocytic property, their substantial and multifaceted contribution to immunological responses and homeostasis i…
 
Functional imaging is a rapidly growing field key to driving new understanding in biology. Insights into the function and interaction of molecules are the key to reveal the underlying cellular mechanisms. In this context, fluorescence lifetime imaging (FLIM) is a powerful tool, providing valuable information beyond spectral imaging. FLIM is immune …
 
In this tutorial on confocal imaging, you will learn how you can: – Capture weaker signals—and still get sound, reproducible data. – Reach faster volumetric imaging without sacrificing resolution – Increase data throughput for your imaging needs Learn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airysc…
 
In this webinar, you will learn: – The complete workflow for in situ cryo-electron tomography – How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) – How mapping these structures back into the native cellular environment reveals new molecular i…
 
In this webinar, you will learn: – How structural biology can change the face of your research – The biological challenges of cancer research – Advantages of cryo-EM for your research Covering both biology and methodology, this webinar will explain how single-particle cryo-electron microscopy enables us to gain insight into cancer development throu…
 
Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and many different labels. In this webinar, we will explain how Elyra 7 with Lattice SIM takes you beyond the diffracti…
 
Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of your array tomography: – Fast and precise trimming of the sample b…
 
In this webinar you will learn: – Improved vitrification: Specimen vitrification without synchronisation fluid – Vitrification strategies: Optimized freezing for different techniques – Light and electrical stimulation: Dissect cellular processes with millisecond precision – A look into the future: freezing of crystals Plunge freezing and cryo imagi…
 
Join Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microscopy (HS-AFM) imaging. In this webinar, you will learn: 1. How CRISPR/Cas9 can be used to “flag” alterations and mutations in DNA, rather than cut it 2. How pairing atomic force microsc…
 
Join Theo Roth as he describes his lab’s novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on HDR. This allows rapid and efficient insertion of large DNA sequences at specific sites in the genomes of primary human T cells, and permits individual or multiplexed modification of…
 
Join us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn: – How to choose the best method to extract your nucleic acids – Which method you should chose to determine nucleic acid quality – How to avoid common pitfalls for both extraction and quality control Virtually all e…
 
In this webinar, you will learn: – How to best prepare your specimen for live cell isolation by laser microdissection – How to optimize your laser microdissection workflow – How to avoid common pitfalls of this technique Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstrea…
 
In this webinar, you will learn: – How to overcome issues with stage drift and crosstalk between channels – How to obtain reliable images and reproducible quantification results – How to proceed with advanced image analysis after restoring images During fluorescence image acquisition, many experimental uncertainties are introduced that affect the c…
 
Join us in this webinar featuring Dr. Omonse Talton who will guide you through developing a “fool proof” enzyme-linked immunosorbent assay (ELISA). In this webinar, you will learn: – When and why you should use the different types of ELISAs– direct, indirect, sandwich, competitive/inhibition ELISA – Major considerations for developing your ELISA – …
 
In this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide mutagenesis screen to identify mutations responsible for resistance to the potent PARP inhibitor talaz…
 
We’ll discuss specific assays in each of these categories, the joys and pitfalls of each assay, and recommendations on how to choose the best method. You will learn tips and strategies for successful assay development using the following methods: Proliferation: 3H-Thymidine Uptake Bromodeoxyuridine Uptake (BrdU) ATP Luminescence Fluorescent Dye Red…
 
While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In this webinar, you will learn: • Low (1-5 genes) vs medium (~300 genes) throughput experimental des…
 
Join us in this webinar featuring Dr. Vicki Doronina as she takes you through vital components of assay design. In this webinar, you will learn: How to choose between immortalized cells and primary cells for your assay How to avoid sources of bias in your cell-based assays How to use high throughput assays, so you can achieve greater reproducibilit…
 
Join us in this webinar featuring Dr. Marlon Stoeckius as he explains how you can improve your single-cell RNA-sequencing (scRNA-seq) experiments. In this tutorial, you will find: -How you can run one scRNA-seq experiment with numerous protein markers in parallel -How you can increase your recovery of single cells (up to four times!) per experiment…
 
qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Generation Sequencing (NGS) has similar potential. However, the presence of large amounts of non-target DNA in most clinical or environmental samples precludes easy and inexpensive analys…
 
Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive observation of the interactions in the living cell is often imp…
 
Elucidating meaningful, unbiased microbial community profiles from complex microbiome samples is challenging. In this webinar, you will learn: – the sources of bias throughout the microbiome analysis workflow – practical solutions for troubleshooting your techniques – new technologies to achieve the most representative and unbiased microbiome profi…
 
Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the ratio of the donor and acceptor intensities (sensitized emission) or by detecting the excited state lifetime of the donor, which decreases…
 
If image analysis is a place you fear to tread, or if you struggle with over complicated and time-consuming microscopy image analysis workflows, this is your opportunity to go beyond those limits. You will learn a fast, efficient and flexible approach to 4D microscopy image analysis, which yields high quality images and results. We’ll cover: • How …
 
Current DNA synthesis and assembly technologies give today’s genetic engineers unprecedented freedom to control every aspect of genetic design. In this webinar on DNA assembly, you will learn: Key concepts in DNA assembly The importance of analyzing combinatorial libraries of genetic designs for natural product biosynthesis Emerging areas of resear…
 
In this webinar, you will learn about the advantages of deconvolving all your images fully automatically. Discussed in this webinar: – How to improve the quality of your images with the new Batch Express – How to increase resolution and signal of all your fluorescent microscopy data – How to deconvolve your new acquired images instantly – If you ar…
 
In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn: The difference and benefits of LASSO cloning over NGS How LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbi…
 
Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as a very small pinhole to increase resolution while the overall detector design delivers better…
 
Join us in this webinar on demystifying DNA assembly. In this webinar, you will learn: – How flanking homology DNA assembly methods work – How to use web-based software to design experimental methods for flanking homology DNA assembly methods – How synthetic DNA fragments fit in to the DNA assembly process Dr. Nathan Hillson will discuss methods in…
 
In this webinar, you will learn about the power of 3D imaging of larger samples. In particular, you will: Gain insight in to how to use x-ray microscopy to analyze biological samples Learn the basics about sample preparation for x-ray microscopy Understand the benefits and drawbacks to different imaging conditions X-Ray Microscopy (XRM) is a relati…
 
An unlikely renaissance is occurring in biological imaging, propelled by the ongoing development of serial blockface electron microscopy imaging technologies. Imaging systems based on scanning EM instruments place large-volume automated EM serial imaging within reach of most labs. How best to take advantage of the automation and versatility? Large …
 
In this webinar, you will learn how to use optical imaging for functional cardiac physiological mapping of transmembrane potential, calcium handling, and metabolism. -The main aspects covered in the webinar include: -How to design an effective optocardiography system and select optimal fluorescence probes for your research needs using open source h…
 
In this tutorial, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease We recently developed a technique that allows localized proteomics of microscopic regions of interest, such as specific cell types o…
 
A major impediment in the determination of high resolution protein structures by single particle cryo-electron microscopy has been the presence of sample heterogeneity. Oftentimes, heterogeneity is due the dynamic nature of protein complexes, which can exist in multiple different conformational states in solution. Recent advancements in cryo-electr…
 
Recent advances in high-throughput multi-beam scanning electron microscopy (EM) and mouse whole-brain EM preparation and collection on tape (“Brain-on-Tape”) have resulted in substantial progress towards a nano-scale map of the whole mouse brain. These maps can be used to determine how individual neurons are synaptically connected and can be used t…
 
In this webinar, you will learn how synthetic biology can be used to engineer desirable and robust behaviors or traits into model organisms. The main aspects to be covered in this webinar include: Introduction to synthetic biology – what it is and what it’s not Popular applications for synthetic biology in research The pros and cons of synthetic bi…
 
Expression and Purification of an Engineered, E. coli-expressed Leishmania donovani Nucleoside Hydrolase with Immunogenic Properties Potential recombinant protein vaccine candidates must meet several criteria: They must be expressed at sufficiently high levels in the organism of choice They must be purified to high purity from the expression system…
 
In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research. The main points in the webinar will include: How to design guide RNAs using online tools specific to the genome and application of interest. Tips and practical advice to assist you in choosing and optimi…
 
Join Dr. Karen O’Hanlon Cohrt for a practical tour of multiplex PCR technology, where you will learn the following and much more: Principle behind multiplex PCR technology Popular applications of this technology How to set up this reaction Advantages and disadvantages Multiplex PCR Can Benefit Your Research Dr O’Hanlon Cohrt will discuss the histor…
 
The rise of open access is changing how research is communicated. In this webinar, we’ll celebrate Open Access Week 2016 by taking a closer look at how open access affects how researchers write and publish their results. Specifically, we will: Define open access and Creative Commons licensing and dive into the numbers about open access (journals, a…
 
In this webinar, you will learn about color reproduction in microscopy images—and how to fix it if your color reproduction goes awry. The main points we will cover are: The reasons that could color reproduction go wrong in your microscopy images How to correct your microscopy images with inappropriate color reproduction How to be ethical with the c…
 
Incorporating New Dyes to Simplify Panel Design: Accelerating Reagent Availability In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components. We will cover: How quality custom reagents in small sizes will make your panel design easier How you can receive these re…
 
In this webinar, you will learn about the process for genotyping cells—particularly tumor cells—and practical advice for analyzing the resulting data. The main points that we will cover are: Practical advice and methods for sample collection and preparation for your genotyping experiments An overview of the available data analysis methods for your …
 
In this webinar you will learn the tips and tricks necessary for you to successfully work with miRNAs, and how you can use them to further your research. The main points we will cover are: Tips and practical advice to help you work with miRNAs, including isolation and delivery The biology and mechanism of miRNAs and why this matters to your researc…
 
Tools for Analyzing Genetic Variants froEvery individual harbors millions of genetic variants, many of which may contribute to phenotype. As the cost of sequencing has plummeted, we now have the opportunity to profile this variation across thousands of individuals. Here I will provide an overview of the workflow to analyze various types of genetic …
 
The current state of the academic job market has come under scrutiny in recent years, largely because of the shrinking number of tenure-track jobs available. This situation has forced graduate students and postdocs to reconsider their employment options. Other scholars may realize that academia is no longer a good fit for them but are unsure of how…
 
Next generation sequencing (NGS) is a powerful tool for investigating genome-wide phenomena. It has been used to study epigenetic profiles as part of the ENCODE project. It is being used to understand the genetic basis of both common and rare diseases. The Cancer Genome Atlas (TCGA) used NGS to map hundreds of cancer genes. There is no area of mode…
 
Whether it’s equipment costing hundreds of thousands of dollars and requiring millions of dollars in R&D investments or standardized kits that relieve you of tedious grunt work day in and day out, you rely on the support of companies like our sponsors to provide you with the equipment and supplies you need. But beyond the things they sell, these co…
 
All mRNA molecules recruit specific proteins to form ribonucleoprotein complexes (mRNPs). Composition and localization of many mRNPs change dynamically from translation to decay. Microscopic techniques with high spatial and temporal resolution are invaluable for studying mRNP biogenesis. We have developed new tools based on fluorogenic forced inter…
 
We know that cellular heterogeneity in the tumor microenvironment complicates the diagnosis and treatment of cancer. Tumors are complex, dynamic systems composed of diverse cell types in various functional states, including cancer cells and infiltrating immune cells. Resolving this cellular heterogeneity requires a single-cell approach, as analyses…
 
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